Title: Utilizing DNA barcodes to assess species diversity for Amphipoda and Polychaeta in Arctic and sub-Arctic waters
|
|
| Biodiversity Institute of Ontario, University of Guelph, Canada | |
Project goals
To generate CO1 DNA barcodes for ~700 specimens of marine invertebrates (Amphipoda and Polychaeta) collected from Igloolik and Resolute. Barcoding these specimens will permit us to further the understand current and future species distributions and limits at sub-Arctic and Arctic locations.
Results (ongoing)
Churchill: Benthic drags, plankton tows and mainly intertidal grabs were taken in summer 2007 and preserved in 95% Ethanol. 87 polychaetes were collected, 60 of those have been sequenced (~70%). 188 amphipods were collected, 110 of those have been sequenced (~60%). We will be returning to Churchill in July 2008 with a focus on polychaetes and amphipods from deeper waters including pelagic specimens.
Resolute and Igloolik: 444 amphipods collected, 318 sequenced (72%); 303 polychaetes collected, 170 sequenced (57%). These specimens are ~10 years old so the success is quite good here (the ones preserved AND frozen work much better). We have a colleague going to Resolute this summer and he will be collecting for us. All specimens have been photographed and submitted to the BOLD database.
